Karine SERRE, PhD
IMMUNOLOGIST
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Latest Posts
21/01/2016
Psoriasis is an autoimmune disease and NOT a chronic inflammatory disease!
12/05/2015
CALL For PAPERS: “Innate T Cell Development and Functions in Immune Diseases”
28/08/2014
XL Annual Meeting of the Sociedade Portuguesa de Imunologia 13-15th October 2014
13/04/2013
Beginnings of breaking down the differentiation program of innate IL-17-production
27/01/2013
The improbable trio NKG2D – γδ T cells – IgE!!
More Posts
Some protocols I have gathered along the years
BrdU BD kit Intracellular FACS staining
Broncheolar Lavages
Buffer list
CFSE staining of T cells
Cresyl Violet Staining for laser-microdissecting lymphoid tissue sections
DNA extraction from mouse ear/tail to genotyping
Experimental Autoimmune Encephalomyelitis (EAE)
In vitro CD4 Th0, Th1, Th2, Th17 and Th22 cell polarizations
Intracellular FACS staining paraformaldehyde with saponin
Isolation of Murine Brain-infiltrating Leukocytes
Isolation of Murine Hepatic Immune cells
Preparation of Agarose Gel and Migration for PCR genotyping
Preparation of Fetal liver cells for reconstituting chimeras
Preparation Paraformaldehyde PAF 4%
Staining for Transcription Factors (e-Bioscience)
Thawing Cryopreserved Cells
Total RNA isolation with High Pure RNA Isolation kit (Roche)
Total RNA isolation with RNeasy MICRO (Qiagen)